Can I use this program with a PLC?

Yes buy connecting the printer cable to the inputs of a PLC, setting the type of output that you need in Help/About Timer Settings to either 5v for On or 5v for Off and then programming the PLC to your particular needs. PLC's are another type of hardware that can be used and are a cheap alternative if to be used for an industrial or commercial application. You need an electrical trade or background if going to use a PLC.